Table 1.
Constitutive and stimulated cytokine production by primary nasal epithelial cells
| |
Stimulant |
|||||
|---|---|---|---|---|---|---|
| Basal |
Staphylococcus aureus PGN |
S. aureus LTA |
Pseudomonas aeruginosa LPS |
CpG | TNF | |
| IL-1β (pg/mL) |
7.1 0–18.7 |
7.7 0–23.8 |
4.2 0–21.9 |
3.6 0–16.4 |
6 0–17.3 |
8.1 0–165 |
| IL-6 (pg/mL) |
29.7 13.7–313 |
140 21.6–695 |
52.1 6.3–459 |
139 7.9–279 |
45 4.7–535 |
956 ** 67.5–3173 |
| IL-8 (pg/mL) |
504 192–1557 |
1363 378–3821 |
663 297–2309 |
740 131–4295 |
520 11.8–2531 |
7817 *** 2033–48 688 |
| IL-10 (pg/mL) |
9.2 4–18.7 |
12.5 4–21.6 |
7.1 0–14.5 |
6.4 0–18.6 |
6.5 0–21.1 |
13 0–67 |
| IL-12 (pg/mL) |
13.2 3.6–19.8 |
12.1 0–21 |
8.8 0–16.1 |
10.3 0–21.4 |
10.4 0–26.7 |
10.4 0–23.3 |
| TNF (pg/mL) |
10 1.7–15 |
6.2 2–24.3 |
7.2 0–11.8 |
6.5 3–16.1 |
6.3 0–17.5 |
† |
Data are expressed as median (upper line, italic) and range (lower line, normal text). n=6 for all conditions. PGN and LTA were applied at 10 μg/mL, LPS at 100 ng/mL, CpG at 1 μM and TNF at 10 ng/mL. Statistical analysis was by Friedman's test and Dunn's post hoc test. *p<0.05, **p<0.01, ***p<0.001 relative to basal levels, by Dunn's post hoc test.
†TNF was used as a positive control; TNF was not measured in TNF-stimulated cells.
IL, interleukin; LPS, lipopolysaccharide; LTA, lipoteichoic acid; TNF, tumour necrosis factor; PGN, peptidoglycan.