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Characterization of siRNA nanomedicine using HSFCM. (a) Bivariate dot-plot of the FL burst area versus the SS burst area for a mixture of 80 nm diameter fluorescent nanoparticles with unstained siRNA-loaded LNPs. (b,c) Representative SS and FL burst traces for empty LNPs (b) and siRNA-loaded LNPs (c). (d) Compiled bivariate dot-plot of the FL burst area versus the SS burst area for empty and siRNA-loaded LNPs.
