Figure 3.

Combination of hypoxia and 24 h cultivation is suitable to augment cellular functions of hPBMNCs. To optimize cell culture conditions for clinical application of hPBMNCs, cells were cultured in different conditions. Then cell survival (A) and VEGF secretion (B) were evaluated in each experimental set, and optimal culture conditions were determined. For both parameters, a combination of hypoxic conditions and culture for 24 h resulted in significantly higher values than other cell culture conditions, whereas all other combinations showed no significant differences. n = 4. **p < 0.05 vs. 20%, 12 h or 20%, 24 h. n.s.: no significant difference.