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. 2014 Oct 11;6(5):570–579.

Figure 4.

Figure 4

Hypoxically preconditioned hPBMNCs improve blood flow in hindlimb ischemia xenograft model. Human PBMNCs were hypoxically preconditioned for 24 h and then grafted into the ischemic right leg of the SCID mouse. In other experimental sets, freshly isolated hPBMNCs, normoxically cultured hPBMNCs, and PBS were injected into ischemic hindlimb. Blood flow was measured by the Laser Doppler Imaging System at day 21 after cell transplantation. (A) Human PBMNCs were prepared from four different healthy volunteers, and each hPBMNC line was transplanted into the ischemic leg (cases 1-4). Left panels indicate representative Laser Doppler images at day 21 after transplantation (case 4). Blood flow was significantly increased in hPBMNC-transplanted legs. Particularly, preconditioned hPBMNCs resulted in higher blood flow compared with any other group (A). Average change in blood flow in cases 1-4 is also shown to be higher in preconditioned hPBMNCs compared with any other group (B). Fr: Freshly isolated hPBMNCs. Nor: Normoxically cultured hPBMNCs. Hyp: Hypoxically preconditioned hPBMNCs. **p < 0.01 vs. PBS; ###p < 0.01 vs. Fr and Nor.