Figure 4.
Cord blood mononuclear cell (MC) P-selectin glycoprotein ligand-1 (PSGL-1) and L-selectin mediate rolling under laminar shear. Cord blood MC and adult peripheral blood mononuclear cells (PBMC) were double stained for CD45 and the indicated adhesion molecules, and analysed using FACS. (a) Representative histograms, (b) percentage of positive cells and (c) mean fluorescence intensities are shown (mean ± SEM; n = 3 to n = 5 different individuals). (d, e) Rolling, (f, g) firm adherence and (h, i) adhesion efficacy of cord and adult blood MC in the flow assay (1 dyn/cm2) was determined in the presence of monoclonal antibodies against PSGL-1, L-selectin, or negative control. The results are (mean ± SEM, n = 4 or 5 independent experiments using MC and activated human umbilical vein endothelial cells from different individuals). (j) Distribution of different leucocyte subpopulations among CD45+ cord blood and adult MC was determined using FACS [percentage of positive cells (mean ± SEM; n = 3 different individuals/group)]. *P < 0·05; **P < 0·01.