Figure 4. Biologic responses to Can f 1-2-4-6 among dog allergic patients.

Allergen-specific basophil degranulation monitored by addition of serial dilutions of rCan f 1 or equimolar amounts of either rCan f 2, rCan f 4, rCan f 6, an equimolar mix or Can f 1-2-4-6 (x-axis) to whole blood from three dog allergic patients. Positivity was defined by analysis of CD63 and CD203c double positive cells (y-axis) using flow cytometry.