Figure 5. Splenocyte proliferation assay in immunized mice.

Proliferative responses after stimulation of splenocytes from immunized mice (immunization allergen denoted as individual graph heading), stimulated with equimolar amounts of rCan f 1, rCan f 2, rCan f 4, rCan f 6, an equimolar mix or Can f 1-2-4-6 (x-axis) and measured by [3H] thymidine incorporation (y-axis). Boxes with median values and horizontal bars denote 50% of values and 1 standard deviation respectively. * p<0.05, ** p<0.01, *** p<0.001 and ns (non significant) p>0.05, analyzed with repeated measurements ANOVA with Tukey's multiple comparison test