Figure 7. Human serum IgE blocking capability of IgG antibodies induced by the linked construct.

Pooled sera from mice (n = 6) immunized with each of rCan f 1, rCan f 2, rCan f 4, rCan f 6, Can f 1-2-4-6 or an equimolar mix was preincubated in wells coated with either rCan f 1, rCan f 2, rCan f 4 or rCan f 6. Sera from multi-sensitized subjects (n = 6) was added to the wells and IgE-responses recorded. The percentage of IgE inhibition is displayed on the y-axis. Median values are indicated in the boxes. The boxes and horizontal bars denote 50% of values and 1 standard deviation respectively.