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. 2014 Oct 29;9(10):e111531. doi: 10.1371/journal.pone.0111531

Table 2. Kinetic parameters for the hydrolysis of the dipeptides AlaPro and AlaPro-NH2 by wild-type and R370E PepQ.

AlaPro-COOH AlaPro-CONH2
kcat/Km(M−1s−1) kcat (s−1) Km (mM) kcat/Km(M−1s−1) kcat (s−1) Km (mM)
WT 1.2×105 139.1±2.5 1.2±0.1 1.2×104 80.7±19.7 6.6±0.5
R370E 5.1×101 6.5±1.1 127±2 2.3×102 1.1±0.4 4.7±0.2