Table 2. Kinetic parameters for the hydrolysis of the dipeptides AlaPro and AlaPro-NH2 by wild-type and R370E PepQ.
AlaPro-COOH | AlaPro-CONH2 | |||||
kcat/Km(M−1s−1) | kcat (s−1) | Km (mM) | kcat/Km(M−1s−1) | kcat (s−1) | Km (mM) | |
WT | 1.2×105 | 139.1±2.5 | 1.2±0.1 | 1.2×104 | 80.7±19.7 | 6.6±0.5 |
R370E | 5.1×101 | 6.5±1.1 | 127±2 | 2.3×102 | 1.1±0.4 | 4.7±0.2 |