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. 2014 Oct 29;9(10):e111177. doi: 10.1371/journal.pone.0111177

Figure 1. Visualization, quantification of DNA damage, and fold change in expression of miR-101 in MCF7 cells.

Figure 1

(a) Fluorescence microscopy where six columns represent - Halo Assay used for calculating NDF values, DAPI staining for nucleus, γH2AX foci with GFP tagged secondary antibodies, RFP-tagged pEP-Vector, Merged-DAPI+GFP-tagged γH2AX and Merged-DAPI+ GFP-tagged γH2AX+ RFP-tagged pEP-Vector; (b) NDF values representing the extent of diffusion of damaged DNA in cells (Halo assay) [27]; (c) number of γH2AX foci; (d) fold change in expression of miR-101, in Controls: mock-pEP-Null-transfected, mock-DMSO treated, and Experimental conditions: pEP-miR-101 transfection and Etoposide 1 µM treatment.