Auxin distribution in the regenerating root tip. A to E, Expression of the auxin-responsive reporter DR5::GUS at 0 h (A), 1 h (B), 24 h (C), 48 h (D), or 72 h (E) after deCAP. Arrowheads in A and B indicate that DR5::GUS expression was reduced in the QC at 1 h (B) compared with 0 h (A) after deCAP. F to J, DR5::GUS expression at 0 h (F), 1 h (G), 24 h (H), 48 h (I), or 72 h (J) after deQC. K to O, DR5::GUS expression at 0 h (K), 1 h (L), 24 h (M), 48 h (N), or 72 h (O) after dePRM. P and Q, DR5::GUS expression in regenerating root tips at 72 h after deCAP and removal of the shoot part (P) or the shoot part together with the maturation zone of the radicle root (Q). R to U, DR5::GUS expression in the root tips at 72 h after deQC (R and S) or dePRM (T and U) and removal of the shoot part (R and T) or the shoot part together with the maturation zone of the radicle root (S and U). Note that auxin accumulation in the tip of damaged roots and regeneration of the root tip was not observed. All images were taken at the same magnification. Bar = 50 μm.