Effect of T-DNA insertion in cPGI on pollen viability and ovule/seed development. A, Bright-field image of Alexander-stained pollen grains from whole-mount filaments. Viable pollen accumulates red stain. Bar = 100 µm. B, Representative siliques from wild-type, cPGI/cpgi-1, and cPGI/cpgi-2 plants. Asterisks mark empty spots in opened siliques. C, Rate of seed abortion/development in cPGI/cpgi
T-DNA mutants and the wild type. Average ± sd (n = 30). Asterisks indicate significant difference to the wild type (Student’s t test, α ≤ 0.01).