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. 1977 Dec;18(3):847–856. doi: 10.1128/iai.18.3.847-856.1977

Serum and tissue lysozyme in leprosy.

T H Rea, C R Taylor
PMCID: PMC421311  PMID: 591069

Abstract

Mean serum lysozyme values were found to be elevated in untreated leprosy patients. Statistically significant elevations were present in each of the three major categories of leprosy, tuberculoid, borderline, and lepromatous. Values were particularly high in patients with severe reversal reactions or Lucio's phenomenon. Prolonged sulfone therapy was associated with a fall in serum lysozyme values. With an immunoperoxidase method to localize lysozyme in leprous tissues, two distinct staining patterns were found, granular and saccular. The grandular pattern of lysozymal staining was found in epithelioid cells and in giant cells, and the intensity of staining showed a positive correlation with serum lysozyme levels. Conversely, a saccular pattern of lysozymal staining was found in lepromatous histiocytes, buth the intensity of staining was unrelated to serum lysozyme levels; the saccular structures contained dense aggregates of Mycobacterium leprae. These two patterns of staining probably represent different functional responses of monocyte-derived granuloma cells, whereas the serum levels reflect, to a varying degree, both the absolute number of such cells and the rate of secretory activity of this cell population as a whole.

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Selected References

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  1. Adams D. O. The granulomatous inflammatory response. A review. Am J Pathol. 1976 Jul;84(1):164–192. [PMC free article] [PubMed] [Google Scholar]
  2. Aquino T. I., Skinsnes O. K. Pathobiologic significance of the subcellular organelles of lepra cells. Int J Lepr Other Mycobact Dis. 1970 Apr-Jun;38(2):134–148. [PubMed] [Google Scholar]
  3. Barnetson R. S., Bjune G., Pearson J. M., Kronvall G. Cell mediated and humoral immunity in "reversal reactions". Int J Lepr Other Mycobact Dis. 1976 Jan-Jun;44(1-2):267–274. [PubMed] [Google Scholar]
  4. COHN Z. A., WIENER E. THE PARTICULATE HYDROLASES OF MACROPHAGES. I. COMPARATIVE ENZYMOLOGY, ISOLATION, AND PROPERTIES. J Exp Med. 1963 Dec 1;118:991–1008. doi: 10.1084/jem.118.6.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Collins M. S., Pappagianis D. Inhibition by lysozyme of growth of the spherule phase of Coccidioides immitis in vitro. Infect Immun. 1974 Sep;10(3):616–623. doi: 10.1128/iai.10.3.616-623.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Currie G. A., Eccles S. A. Serum lysozyme as a marker of host resistance. I. Production by macrophages resident in rat sarcomata. Br J Cancer. 1976 Jan;33(1):51–59. doi: 10.1038/bjc.1976.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Currie G. A. Serum lysozyme as a marker of host resistance. II. Patients with malignant melanoma, hypernephroma or breast carcinoma. Br J Cancer. 1976 Jun;33(6):593–599. doi: 10.1038/bjc.1976.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dobbins J. W., Binder H. J., Spiro H. M., Finch S. C. Serum lysozyme in inflammatory bowel disease. Gastroenterology. 1976 Mar;70(3):469–471. [PubMed] [Google Scholar]
  9. Falchuk K. R., Perrotto J. L., Isselbacher K. J. Serum lysozyme in Crohn's disease and ulcerative colitis. N Engl J Med. 1975 Feb 20;292(8):395–397. doi: 10.1056/NEJM197502202920805. [DOI] [PubMed] [Google Scholar]
  10. Gilbert H. S., Weinreb N. Increased circulating levels of transcobalamin ii in gaucher's disease. N Engl J Med. 1976 Nov 11;295(20):1096–1101. doi: 10.1056/NEJM197611112952002. [DOI] [PubMed] [Google Scholar]
  11. Goldstein I. M., Horn J. K., Kaplan H. B. Letter: Hypercalcemia: a possible cause for elevated serum lysozyme levels. Ann Intern Med. 1976 Apr;84(4):445–446. doi: 10.7326/0003-4819-84-4-445. [DOI] [PubMed] [Google Scholar]
  12. Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hansen N. E., Karle H., Andersen V., Olgaard K. Lysozyme turnover in man. J Clin Invest. 1972 May;51(5):1146–1155. doi: 10.1172/JCI106907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hylander E., Hansen N. E., Karle H., Janrum S. Serum lysozyme in Crohn's disease and ulcerative colitis. Scand J Gastroenterol. 1976;11(2):213–217. [PubMed] [Google Scholar]
  15. Imaeda T. Electron microscopy. Approach to leprosy research. Int J Lepr. 1965 Jul-Sep;33(3 Suppl):669–688. [PubMed] [Google Scholar]
  16. Klockars M., Adinolfi M. C., Osserman E. F., Furth J. Ontogeny of lysozyme in the rat. Proc Soc Exp Biol Med. 1974 Feb;145(2):604–609. doi: 10.3181/00379727-145-37859. [DOI] [PubMed] [Google Scholar]
  17. Klockars M., Petterson T., Riska H., Hellström P. E. Pleural fluid lysozyme in tuberculous and non-tuberculous pleurisy. Br Med J. 1976 Jun 5;1(6022):1381–1381. doi: 10.1136/bmj.1.6022.1381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Klockars M., Roberts P. Stimulation of phagocytosis by human lysozyme. Acta Haematol. 1976;55(5-2):289–295. doi: 10.1159/000208029. [DOI] [PubMed] [Google Scholar]
  19. Lieberman J., Beutler E. Elevation of serum angiotensin-converting enzyme in Gaucher's disease. N Engl J Med. 1976 Jun 24;294(26):1442–1444. doi: 10.1056/NEJM197606242942609. [DOI] [PubMed] [Google Scholar]
  20. Lieberman J. Elevation of serum angiotensin-converting-enzyme (ACE) level in sarcoidosis. Am J Med. 1975 Sep;59(3):365–372. doi: 10.1016/0002-9343(75)90395-2. [DOI] [PubMed] [Google Scholar]
  21. MYRVIK Q., WEISER R. S. A tuberculostatic serum substance possessing lysozyme-like properties. Am Rev Tuberc. 1951 Dec;64(6):669–674. doi: 10.1164/art.1951.64.6.669. [DOI] [PubMed] [Google Scholar]
  22. Mason D. Y., Farrell C., Taylor C. R. The detection of intracellular antigens in human leucocytes by immunoperoxidase staining. Br J Haematol. 1975 Nov;31(3):361–370. doi: 10.1111/j.1365-2141.1975.tb00867.x. [DOI] [PubMed] [Google Scholar]
  23. Mason D. Y., Taylor C. R. The distribution of muramidase (lysozyme) in human tissues. J Clin Pathol. 1975 Feb;28(2):124–132. doi: 10.1136/jcp.28.2.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. McClelland D. B., Shearman D. J., Lai A Fat R. F., van Furth R. In vitro synthesis of immunoglobulins, secretory component, complement and lysozyme by human gastrointestinal tissues. II. Pathological tissues. Clin Exp Immunol. 1976 Jan;23(1):20–27. [PMC free article] [PubMed] [Google Scholar]
  25. McClelland D. B., van Furth R. In vitro synthesis of lysozyme by human and mouse tissues and leucocytes. Immunology. 1975 Jun;28(6):1099–1114. [PMC free article] [PubMed] [Google Scholar]
  26. Myrvang B., Godal T., Ridley D. S., Fröland S. S., Song Y. K. Immune responsiveness to Mycobacterium leprae and other mycobacterial antigens throughout the clinical and histopathological spectrum of leprosy. Clin Exp Immunol. 1973 Aug;14(4):541–553. [PMC free article] [PubMed] [Google Scholar]
  27. Nugent F. W., Mallari R., George H., Ridley N. Serum lysozyme in inflammatory bowel disease. Gastroenterology. 1976 Jun;70(6):1014–1016. [PubMed] [Google Scholar]
  28. OSHIMA S., MYRVIK Q. N., LEAKE E. The demonstration of lysozyme as a dominant tuberculostatic factor in extracts of granulomatous lungs. Br J Exp Pathol. 1961 Apr;42:138–144. [PMC free article] [PubMed] [Google Scholar]
  29. Osserman E. F., Lawlor D. P. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 1966 Nov 1;124(5):921–952. doi: 10.1084/jem.124.5.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ota H., Osserman E. F. Hydrolytic enzymes of human blood monocytes and neutrophils with special reference to lysozyme (muramidase). Tohoku J Exp Med. 1972 Jul;107(3):229–240. doi: 10.1620/tjem.107.229. [DOI] [PubMed] [Google Scholar]
  31. Ota H., Yasuma A. Lysozyme activity in hematologic and non-hematologic disorders with special reference to reactive monocytosis associated with chronic infections and inflammatory reactions. Tohoku J Exp Med. 1974 Sep;114(1):15–26. doi: 10.1620/tjem.114.15. [DOI] [PubMed] [Google Scholar]
  32. Pascual R. S., Gee J. B., Finch S. C. Usefulness of serum lysozyme measurement in diagnosis and evaluation of sarcoidosis. N Engl J Med. 1973 Nov 15;289(20):1074–1076. doi: 10.1056/NEJM197311152892007. [DOI] [PubMed] [Google Scholar]
  33. Perillie P. E., Kaplan S. S., Finch S. C. Significance of changes in serum muramidase activity in megaloblastic anemia. N Engl J Med. 1967 Jul 6;277(1):10–12. doi: 10.1056/NEJM196707062770103. [DOI] [PubMed] [Google Scholar]
  34. Perillie P. E., Khan K., Finch S. C. Serum lysozyme in pulmonary tuberculosis. Am J Med Sci. 1973 Apr;265(4):297–302. doi: 10.1097/00000441-197304000-00005. [DOI] [PubMed] [Google Scholar]
  35. Pounder R. E., Avella J. R., McCallum H., Misiewicz J. J. Letter: Serum lysozyme in inflammatory bowel disease. Lancet. 1975 Aug 2;2(7927):228–229. doi: 10.1016/s0140-6736(75)90696-0. [DOI] [PubMed] [Google Scholar]
  36. Pruzanski W., Saito S., Ogryzlo M. A. The significance of lysozyme (muramidase) in rheumatoid arthritis. I. Levels in serum and synovial fluid. Arthritis Rheum. 1970 Jul-Aug;13(4):389–399. doi: 10.1002/art.1780130405. [DOI] [PubMed] [Google Scholar]
  37. Rea T. H., Levan N. E. Erythema nodosum leprosum in a general hospital. Arch Dermatol. 1975 Dec;111(12):1575–1580. [PubMed] [Google Scholar]
  38. Ridley D. S., Jopling W. H. Classification of leprosy according to immunity. A five-group system. Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255–273. [PubMed] [Google Scholar]
  39. Ridley D. S. Reactions in leprosy. Lepr Rev. 1969 Apr;40(2):77–81. doi: 10.5935/0305-7518.19690016. [DOI] [PubMed] [Google Scholar]
  40. Ridley D. S., Waters M. F. Significance of variations within the lepromatous group. Lepr Rev. 1969 Jul;40(3):143–152. doi: 10.5935/0305-7518.19690026. [DOI] [PubMed] [Google Scholar]
  41. Senn H. J., Chu B., O'Malley J., Holland J. F. Experiemental and clinical studies on muramidase (lysozyme). I. Muramidase activity of normal human blood cells and inflammatory exudates. Acta Haematol. 1970;44(2):65–77. doi: 10.1159/000208664. [DOI] [PubMed] [Google Scholar]
  42. Sheskin J., Convit J. Results of a double blind study of the influence of thalidomide on the lepra reaction. Int J Lepr Other Mycobact Dis. 1969 Apr-Jun;37(2):135–146. [PubMed] [Google Scholar]
  43. Skinsnes O. K., Matsuo E., Chang P. H., Andersson B. In vitro cultivation of leprosy bacilli on hyaluronic acid based medium. 1. Preliminary report. Int J Lepr Other Mycobact Dis. 1975 Jul-Sep;43(3):193–203. [PubMed] [Google Scholar]
  44. Unanue E. R. Secretory function of mononuclear phagocytes: a review. Am J Pathol. 1976 May;83(2):396–418. [PMC free article] [PubMed] [Google Scholar]
  45. Weinberg R. S., Tessler H. H. Serum lysozyme in sarcoid uveitis. Am J Ophthalmol. 1976 Jul;82(1):105–108. doi: 10.1016/0002-9394(76)90673-5. [DOI] [PubMed] [Google Scholar]
  46. Zucker S., Hanes D. J., Vogler W. R., Eanes R. Z. Plasma muramidase: a study of methods and clinical applications. J Lab Clin Med. 1970 Jan;75(1):83–92. [PubMed] [Google Scholar]

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