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. 2014 Aug 7;166(2):853–868. doi: 10.1104/pp.114.242560

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© 2014 American Society of Plant Biologists. All Rights Reserved.

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Figure 5. — Interaction between AtTIM9 and AtTIM10 proteins. A, Yeast two-hybrid assay showing interaction between AtTIM9 and AtTIM10. The 10-fold serial dilutions were plated onto lacking media. AD, pGADT7 vector; BK, pGBKT7 vector; Ade, adenine; SD, synthetic dextrose. B, Coimmunoprecipitation (IP) assay showing the interaction between AtTIM9 and AtTIM10 in N. benthamiana leaves in vivo. C, BiFC assay showing the interaction between AtTIM9 and AtTIM10 directly in Arabidopsis living protoplasts. YC, YFP C-terminal fragment (aa156-239); YN, YFP N-terminal fragment (aa1-155). Bars = 10 μm. [See online article for color version of this figure.]