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. 2014 Aug 22;166(2):889–902. doi: 10.1104/pp.114.246314

Figure 4.

Figure 4.

Analysis of vcc embryos. A, Mature wild-type (WT) and vcc-2 cotyledons were stained with Perls/DAB (Roschzttardtz et al., 2009) to detect iron accumulation in the proendodermis that surrounds procambial strands. Vein disconnections or gaps (arrowheads) were evident in the distal areoles of vcc cotyledons. B to E, Representative examples of GFP expression patterning in wild-type Col-0 and vcc-2 embryos expressing pDR5:GFP. B and C, Torpedo embryo stage. D and E, Dissected cotyledons from bent cotyledon stage embryos. S, Suspensor. Bars = 20 μm.