Figure 7.
Histological characterization of vasculature in cotyledons. A and B, Median longitudinal optical sectioning of a Col-0 cotyledon (A) and its corresponding transversal sectioning (B) showing complete division of the procambium into phloem and xylem initials. C, Median longitudinal section of an ops-4 cotyledon showing a gap in the vascular networks (arrowheads). D and E, Trans-section (D) and lateral section (E) in the distal loop in the positions indicated by the asterisks showing a progressive loss of procambial divisions. F, Median longitudinal sectioning of a vcc-3 cotyledon. The green line indicates the vascular loop. G, Curved lateral section of the corresponding loop indicated by the green line in G. H to J, Three different trans-sections at different positions of the green line, from proximal (a) to median (b; yellow dot) to distal (c) positions. K, Median longitudinal section of a vcc-3 ops-4 cotyledon. L, Curved lateral section of the corresponding loop through the green line in K. M to O, Three different trans-sections at different positions of the green line, from proximal (a) to median (b; yellow dot) to distal (c) positions. Ab, Abaxial; LV, lateral vein; MV, middle vein. Bars = 10 μm.