Figure 8.

Effect of salt stress on the transcript level of OsHAK5 in rice. Ten-day-old rice seedlings (cv Nipponbare) were grown in IRRI solution for 2 weeks, then transferred to a control solution without salt (0 mm NaCl; −Na) or to a salt-enriched solution (100 mm NaCl; +Na) for the indicated durations. Total RNA was isolated from roots, basal node and sheath, and leaf blades of the seedlings, and OsHAK5 transcript levels were determined by qRT-PCR. A housekeeping gene, ACTIN (OsRac1; accession no. AB047313), was used as an internal standard. Error bars indicate se (n = 3 biological replicates). All primers are listed in Supplemental Table S2.