Figure 2.

Coexpression of PLIM2a Suppressed Pollen Tube Blebbing Caused by LePRK1 Overexpression, and Coexpression of KPP Restored Pollen Tube Blebbing.

(A) to (H), (J), and (L) to (R) Representative tobacco pollen tubes transiently expressing the denoted constructs or construct combinations. All genes were driven by the LAT52 promoter. Arrows point to blebs. The dotted line in (B) outlines the margin of the pollen tube tip. Bars = 20 μm.

(I) and (K) Measurements of pollen tubes in transient assays after 4 to 6 h of cultivation. Asterisks indicate a significant difference from the mRFP or eGFP control (P < 0.01, Student’s t test). n = 3 independent experiments. Error bars indicate se.