Figure 3.

Tomato PLIM2a Promotes F-Actin Bundling in Vitro and Interacts with KPP.

(A) and (B) High-speed (A) and low-speed (B) cosedimentation assays for in vitro actin bundling. PEL, pellet fraction; SUP, supernatant fraction.

(C) Direct visualization of actin bundles induced by PLIM2a at pH 6.8. Bars = 20 μm.

(D) Low-speed cosedimentation assay for in vitro actin bundling of the KPP and PLIM2a combination at two different Ca²⁺ concentrations at pH 7.4. The left panel shows a representative experiment; the chart at right shows a statistical analysis of the relative amount of actin from five independent experiments.

(E) In vitro pull-down assays. His-PLIM2a can be coprecipitated with GST-KPP under high-CaCl₂ concentrations.