Figure 3.

The Arabidopsis RNase H2 Complex Comprises Three Subunits.

(A) and (B) Protein-protein interaction between different RNase H2 subunits as identified by tandem affinity purification from cell cultures cultivated in the absence (A) or presence of 10 mM HU for 24 h (B). Arrows point from bait to prey and correspond to interactions that were confirmed in a repeat experiment (Supplemental Data Set 2).

(C) Yeast two-hybrid interactions between the different subunits of RNase H2. The GUS protein was used as negative control.