Figure 5.

Lack of RNase H2 Activity Triggers the Accumulation of γ-H2AX Foci.

(A) Average number of γ-H2AX foci per nucleus of wild-type (Col-0) and wee1-1 mutants, untreated or treated with HU. Data represent mean ± sd (n = 100, *P value < 0.05, two-sided Student’s t test).

(B) Detection of γ-H2AX foci in wild-type (Col-0), wee1-1, trd1-2, and wee1-1 trd1-2 root tip cells untreated (−HU) or treated with 1 mM HU (+HU). Bar = 2 μm.

(C) γ-H2AX immunofluorescence in S- or early G2-phase nuclei of trd1-2 mutants (being Edu positive). Bar = 2 μm.