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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Nat Neurosci. 2014 Oct 5;17(11):1528–1535. doi: 10.1038/nn.3831

Fig. 5. Displaced RGPs remain proliferative.

Fig. 5

(a) Representative images of E15.5 WT and Sas-4−/− p53−/− cortices stained with the antibodies against PAX6 (green) and Ki67 (red), a cell proliferation marker, and DAPI (blue). Note that similar to RGPs in the VZ of the WT cortex, nearly all RGPs in the Sas-4−/− p53−/− cortex, regardless of their localization in the VZ (area 1) or outside the VZ (area 2), are positive for Ki67, indicating that they remain proliferative. Scale bars: 50 μm and 20 μm. (b, d) Representative images of E15.5 WT and Sas-4−/− p53−/− cortices stained with the antibodies against PHH3 (red, b) or P-VIMENTIN (green, d) that labels mitotic cells and DAPI (blue). Note the increase in the number of mitotic cells in the Sas-4−/− p53−/− cortex. Scale bar: 50 μm. (c, e) Quantification of the number of PHH3+ (c) or P-VIMENTIN+ (e) cells per unit area (WT, n=5; Sas-4−/− p53−/−, n=5). **, p<0.01. Data are presented as mean ± SD. Individual p values and degrees of freedom are available in the Supplementary Methods Checklist.