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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Nat Neurosci. 2014 Oct 5;17(11):1528–1535. doi: 10.1038/nn.3831

Fig. 6. Centrosome loss causes mitotic delay.

Fig. 6

(a) Representative images of mitotic cells at the VZ surface (top) and Extra-VZ (bottom) in E15.5 WT and Sas-4−/− p53−/− cortices stained for P-VIMENTIN (green) and DAPI (blue). Closed arrowheads indicate cells in pro/pro-metaphase, and open arrowheads indicate cells in meta/ana/telophase. High magnification images of pro/pro-metaphase (labeled as 1) and meta/ana/telophase (labeled as 2) are shown to the right. Note that the Sas-4−/− p53−/− cortex contains more cells in pro/pro-metaphase compared to WT control. Scale bars: 25 μm and 2 μm. (b) Quantification of the fraction of mitotic cells in pro/pro-metaphase (black) versus in meta/ana/telophase (white), which reflects mitotic progression (WT, 461 cells from n=3 brains; Sas-4−/− p53−/−, 1,366 cells from n=3 brains). **, p<0.01. (c) Representative images of mitotic cells labeled by PHH3 (red) at the VZ surface and away from the VZ surface (Extra-VZ) in E15.5 WT and Sas-4−/− p53−/− cortices stained for PCNT (green), a centrosomal marker, and DAPI (blue). Note that while WT mitotic cells possess two PCNT foci, mitotic cells at the VZ surface (top) and in the Extra-VZ (bottom) of the Sas-4−/− p53−/− cortex largely contain either none or one PCNT focus. Scale bar: 2 μm. (d) Quantification of the number of PCNT foci in mitotic cells at the VZ surface or Extra-VZ (WT, 226 cells from n=3 brains; Sas-4−/− p53−/−, 690 cells from n=3 brains). **, p<0.01. Data are presented as mean ± SD of individual brains. Individual p values and degrees of freedom are available in the Supplementary Methods Checklist.