Figure 6.

Lysosome Ca²⁺ release in response to ROS in living CAMs. Glycyl-l-phenylalanine-β-naphthylamide (GPN, 200 μM) was used to release Ca²⁺ from lysosomes. Representative lysosomal Ca²⁺ traces (A) and summarized data showing the effects of NAADP antagonists, PPADS (50 μM) or NED-19 (10 μM) on GPN-induced lysosomal Ca²⁺ release in CAMs treated with X/XO or H₂O₂ (B) or Nox1 cDNA (C; n = 6 for all panels). *P < 0.05 versus Ctrl or Scramble; ^#P < 0.05 versus CAMs treated with X/XO or H₂O₂ or Nox1 cDNA transfection alone.