Figure 7.

Confocal microscopic detection of Ca²⁺ release from lysosomes locally in CAMs. (A) Representative confocal microscopy images showing Ca²⁺ release regions that co-localized with lysosomes as shown by yellow spots formed by green fluo-4 signals with rhodamine-red lysosomal marker (Lyso/Rho). Summarized data showing the co-localization coefficient of Ca²⁺/fluo-4 with Lyso/Rho signals in CAMs treated with X/XO or H₂O₂ (B) or Nox1 cDNA (C; n = 6 for all panels). *P < 0.05 versus Ctrl or Scramble; ^#P < 0.05 versus CAMs treated with X/XO or H₂O₂ or Nox1 cDNA transfection alone.