Figure 4.

Effect of proinflammatory cytokines and thapsigargin on ribosomal occupancy of Actb, Tbp, Ccnd1, and Bip. MIN6 β cells were untreated (CTL), treated with proinflammatory cytokines (Cyto) for 24 hours, or treated with thapsigargin (Tg) for 4 hours and then were subjected to PRP analysis with fractionation of the sedimentation gradient. The percentage of each mRNA species (indicated above each bar graph) residing in polyribosomes is shown. Data represent means ± SEM (n = 3–5). *, P < .05 compared to the control.