Figure 6.

Chloroquine only modestly affects GH-induced GHR-GHR complementation. γ2A-JAK2-GHR-Nluc cells transiently expressing GHR-Cluc were treated with GH (500 ng/mL) alone or in the presence of chloroquine (100 μM), as indicated, after basal bioluminescence was determined. Bioluminescence was measured serially thereafter over 40 minutes. Data are expressed as mean ± SE of GH-induced signal as a percentage above baseline signal (n = 3 per condition). A value of P < .05 was attained at 30 minutes, 35 minutes, and 40 minutes. The figure shown is representative of three such experiments.