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. 2014 Oct 25;14:265. doi: 10.1186/s12866-014-0265-8

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© Rifat et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mtb senX3 and regX3 are required for optimal growth during P _i depletion, but not in P _i -replete broth. The wild-type, senX3::Tn, senX3::Tn Comp, regX3::Tn, and regX3::Tn Comp strains were each cultured in reconstituted 7H9 broth containing 25 mM P_i (A) and P_i-depleted broth (50 μM P_i) (B). Each experiment was repeated twice with similar values of optimal density (O.D.600_nm).