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. 2014 Oct 25;14:265. doi: 10.1186/s12866-014-0265-8

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© Rifat et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Both senX3 and regX3 are required for Mtb survival during nutrient starvation, although there appears to be a senX3 -independent contribution of regX3 . The wild-type CDC1551, senX3::Tn, senX3::Tn Comp, regX3::Tn, and regX3::Tn Comp were subcultured in 1xPBS with 0.05% Tween-80 and CFU were counted at different time points after plating the diluted cultures on Middlebrook 7H10 plates (mean ± SD) and incubating for 21 days. Triplicate samples were used in the experiment and the experiment was repeated twice under the same condition. *p <0.05; **p <0.01.