Table 2.
List of primers used in this study
| Primer | Sequence (5’—3’) | Purpose of amplification |
|---|---|---|
| dr001-F | CCCTCTAGAACGAAATCGTCGGACTGAAC | senX3-regX3 operon and flanking sequence |
| dr002-R | CCCTCTAGAAGGTGAGATCGCCAAGCTAC | |
| senX3-F | CCGAGTTGATCGAGCTATCC | senX3 probe and gene expression |
| senX3-R | AGTGCGGTAACCAGCAGAGT | |
| dl15-F | GCTGACGACTACGTGACCAA | regX3 probe |
| dl16-R | CGACGAGCAGCTTTGTCC | |
| kan-F | CTGTGCTCGACGTTGTCACT | kanamycin gene |
| kan-R | AGCCAACGCTATGTCCTGAT | |
| hyg4-F | AGGTCTTCCCGGAACTGCTG | hygromycin gene |
| hyg4-R | TCCTCGAACACCTCGAAGTC | |
| regX3-F | TGTTGATTGTGGAGGACGAG | gene expression |
| regX3-R | CGCAACTGCTTGCATACATC | |
| pstC2-F | GTCGATCATCTTCGGGTTGT | gene expression |
| pstC2-R | ATTTGGATCAGCGGAGTCTG |