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. 2014 Oct 23;14:409. doi: 10.1186/1472-6882-14-409

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© Bonvicini et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Representative dose–response curve of a Candida albicans clinical isolate. Experimental IC₅₀ values were obtained by the interpolation of data on the specific dose–response curve, generated by means of a nonlinear regression analysis. In particular, the reduction of the yeast growth, expressed as percentage, was plotted against the drug concentration, in logarithm scale, leading to the generation of a sigmoidal curve following the equation Y = 100/(1 + 10^((LogIC50-X)*HillSlope))), where Y =50.