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. 2014 Oct 24;13:240. doi: 10.1186/1476-4598-13-240

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© Pang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of Pim-1 on cell growth and cell cycle progression in NSCLC cells in vitro . (A) A549 and H1299 cells were transfected with Pim-1 siRNA at a final concentration of 50nM. Pim-1 expression was detected using qRT-PCR (Top) and Western blot (Bottom) at 48 h posttransfection. (B) A549 and H1299 cells were transfected as in (A). In the indicated time periods (0-96 h) posttransfection, cell viability was evaluated using MTT assay. (C) Cells were stained using propidium iodide (PI) 48 h posttransfection and the cell cycle distribution was analyzed by FACS. (D) CyclinD1 and CDK4 protein expressions were detected in A549 and H1299 cells after transfection with si-Pim-1 by Western Blot assay. All data were obtained from three independent experiments and shown as mean ± SD and *P <0.05.