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. 2014 Oct 23;15(1):921. doi: 10.1186/1471-2164-15-921

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© Lam et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NGS data and quantitative real-time PCR of representative genes encoding inorganic ion channel or transporters that were identified as differentially expressed i.e. (A) abundant in freshwater (FW) gill or (B) abundant in seawater (SW) gill of Oreochromis mossambicus . Data that assume normality and homogeneity of variance were subjected to ANOVA followed by Tukey-Kramer post-hoc test. Data that do not assume normality or homogeneity of variance were subjected to Kruskal Wallis followed by Mann–Whitney U test and Bonferonni correction for multiple comparisons. **indicates P-value < 0.01 while *indicates P-value < 0.05 when SW and HSW are compared to FW for the same gene. N = 6–7 replicates.