Table 1.
BLASTX of combined FW&SW assembled contigs mapped to Oreochromis niloticus , Danio rerio and Homo sapiens protein databases
| Database | BLASTX E-value range | Number of mapped contigs (Percentage of total selected contigs)* | Number of mapped unique identifier in protein database (Percentage of sequences in respective database)** |
|---|---|---|---|
| Oreochromis niloticus (NCBI Nile tilapia protein database release 101 containing 45443 sequences)** | 0 | 9845 (12.8%) | 8830 (19.4%) |
| ≤1E-100 | 15733 (20.4%) | 12946 (28.5%) | |
| ≤1E-50 | 23556 (30.6%) | 16433 (36.2%) | |
| ≤1E-20 | 32541 (42.2%) | 18474 (40.7%) | |
| ≤1E-05 | 38925 (50.5%) | 19510 (42.9%) | |
| Danio rerio (NCBI zebrafish protein database release 103 containing 42940 sequences)** | 0 | 5667 (7.35%) | 4989 (11.6%) |
| ≤1E-100 | 10410 (13.51%) | 8594 (20.0%) | |
| ≤1E-50 | 16452 (21.35%) | 12133 (28.3%) | |
| ≤1E-20 | 24150 (31.34%) | 14779 (34.4%) | |
| ≤1E-05 | 31775 (41.23%) | 16397 (38.2%) | |
| Homo sapiens (Direct BLASTX to the NCBI human non-redundant database)** | 0 | 4418 (5.7%) | 4063 |
| ≤1E-100 | 8583 (11.1%) | 7796 | |
| ≤1E-50 | 14018 (18.2%) | 12317 | |
| ≤1E-20 | 20785 (27.0%) | 17022 | |
| ≤1E-05 | 28446 (36.9%) | 21075 |
*Total selected contigs (average coverage ≥20) =77062. Percentage of total selected contigs = (number of contigs/77062) x 100%.
**Percentage of Nile tilapia protein database = (Number of unique identifiers/45443) x 100%. Percentage of zebrafish protein database = (Number of unique identifiers/42940) x 100%. Direct BLASTX to the NCBI human non-redundant database were used and information on exact number of sequence is not available, therefore percentage of human nr database is not determined.