. 2014 Oct 21;6(10):3893–3906. doi: 10.3390/v6103893

Table 1.

List of primers used in the multiplex detection for PRSV, PLDMV, and PapMV.

Virus	Primer ^a	Sequence (5’-3’)	Size (bp)	Target Gene	Location (nt) ^b
PRSV	PRSV953-F	GCGATGCTCATAAACATACCTGA	953	HC-Pro/P3	2774–2796
	PRSV953-R	TGTACACAGTACTTCGGTGAGAAGTCGTA	953	HC-Pro/P3	3698–3726
	PRSV613-F	TTGTGTACGACTTCTCACCGAA	613	P3	3693–3714
	PRSV613-R	CGAATGTCATCCAAAGACTGATGATAAAC	613	P3	4277–4305
	PRSV515-F	TTGTGTACGACTTCTCACCGAA	515	P3	3693–3714
	PRSV515-R	CACATCAATCATCATCAAAATTAATGT	515	P3	4181–4207
PapMV	PapMV476-F	ATGGTAGCTGCTAAGGTTCCAGC	476	CP	6024–6046
	PapMV476-R	GACCCAGAAATTTGGCCTTTGGTGATG	476	CP	6473–6499
	PapMV205-F	CCAAATTTGCCGCGTTCGACT	205	CP	6295–6315
	PapMV205-R	GACCCAGAAATTTGGCCTTTGGTGATG	205	CP	6473–6499
PLDMV	PLDMV355-F	GGCATGTGGTTTATGATGCAAGGG	355	CP	9528–9551
PLDMV	PLDMV355-R	GCTCCGTGTTCTCAGTCGCATT	355	CP	9861–9882

^a F, sense primer; R, antisense primer. ^b The targeting nucleotide locations according to the complete genome sequence of PRSV (HQ424465), PapMV (JX524226), and PLDMV (JX974555).