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. 2014 Oct 26;15:49. doi: 10.1186/s12865-014-0049-9

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© Goupil et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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qRT-PCR analysis of signature genes of CD4+ T-cell subsets polarized in vitro . Naive CD4+ precursors were harvested from CD4C/HIV^MutA Tg and non-Tg mice, and incubated with subset-specific differentiating cytokines and blocking antibodies. Bars represent the mean ± standard error range of significantly (p < 0.05) up- or down-regulated genes compared to that of control naive cells from non-Tg mice, incubated without cytokines and antibodies. In all differentiating conditions, gene expression of IL-4 and IL-10 was not significantly different (p > 0.05) from control. Data are from 6 independent experiments.