Figure 10.

Binding of YscU_CN and variants to vesicles. The binding capacity of YscU_CN variants to vesicles with varying anionic charge density was probed by observing the CD signal at 220 nm. CD spectra were recorded at 20°C in 5 mM sodium phosphate, 30 mM NaCl, and 1 mM TCEP at pH 6.0. The data were analyzed with a one-site binding model (solid lines) and the binding capacity is judged from the maximum amplitude of the CD signal. Black, CN_WT; green, CN₆; red, CN_R223A; purple, CN_K218A; blue, CN_E220A.