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YscUC interacts with negatively charged phospholipid vesicles. The interaction between YscUC and vesicles was probed with far-UV CD spectroscopy in a buffer consisting of 10 mM sodium phosphate at pH 7.4 and a temperature of 37°C. CD spectra were acquired in buffer (black) in the presence of DMPC (zwitterionic; dashed light blue) or DMPG vesicles (anionic; violet). Addition of 100 mM KCl (magenta) attenuated the interaction between YscUC and DMPG vesicles.
