Figure 3.
YscUCN undergoes a coil-to-helix transition upon binding to negatively charged phospholipid vesicles. The interaction between YscUCN and different membrane models was probed with far-UV CD as in Fig. 2. (A) The YscUCN interaction with negatively charged phospholipid vesicles (DMPG) is proportional to negative charge density. (B) KCl attenuates the YscUCN interaction with DMPG vesicles in a dose-dependent manner. (C) YscUCN interacts with SDS micelles (purple) and E. coli IM lipid extracts (light blue) with a CD response similar to that observed with DMPG vesicles.