Figure 5.

NMR identification of helical segments of YscU_CN in complex with SDS micelles. (A) Deviation (Δδ) of observed ¹³C^α chemical shifts from random-coil values are displayed against the primary sequence of YscU_CN. Gray horizontal bars indicate α-helical regions identified by the program MICS (35), with darker tone indicating rigid helices (see Supporting Material). (B) ³J_HNHα couplings in SDS-bound YscU_CN. Horizontal lines indicate standard cutoffs (48) used during classification of backbone conformations (<6 Hz, α-helical (dash-dotted gray line); >8 Hz, extended (dashed gray line)).