Figure 3.

(A) Direct comparison between the time-dependent edge position (black) and velocity (green). The decay of the correlation is given in the autocorrelation plot of Fig. S3 in the Supporting Material. (B) Tension (bleb panel A) resulting from the VAR method (blue) and the PSD method (red) is presented. The tension measured by different methods is consistent and suggests a systematic increase over time. The growth phase (light-brown background) is defined by the growth arrest. (C) Scatter plot of the data presented in panel B, where the markers are color codes for the edge velocity. (D) Histogram of the velocities found in the data shown in panel A. This is used to determine whether there are different phases during bleb growth that are marked by different velocities. In this example, we can identify three different growth phases that are indicated by the three peaks at positive velocities. (E) Distribution of the measured tension for all blebs and all the time-points. The distribution shows a large variation starting at 5 pN/μm up to almost 600 pN/μm. Again, the different methods show consistent values. (F) To finally check whether the two measurements are indeed consistent, we also analyzed the data using a scatter plot, where all obtained experimental values are shown. Again, the edge velocity was used as the color coding. This plot confirms that the two methods provide consistent values, inasmuch as most points lie on the diagonal (dashed line). The highest tensions typically correspond to the low velocity. The color bar of panels C and F is equivalent.