Figure 4.

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed to detect cell death among various capsaicin-exposed bovine bone marrow mesenchymal stem cell (BMSC) groups. Representative TUNEL assay images in the cells are shown (×100). First row: capsaicin treatment for 2 days, second row: capsaicin treatment for 4 days, third row: capsaicin treatment for 6 days. Cell death caused by capsaicin exposure was indicated by red coloring. BMSCs were stained with fluorescein-labeled dUTP according to the protocols described above.