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. 2014 Sep 4;7(11):1275–1286. doi: 10.1242/dmm.014720

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Fate of proglucagon-gene-expressing intestinal cells explored by lineage tracing. Immunohistochemistry of frozen sections using an anti-RFP antibody and confocal microscopy images of polyp, duodenum and colon from wild-type (‘WT’; M-P,Y-Zii), GluLKB1^fl/+ (I-L,U-X) and GluLKB1KO (A-H,Q-T) mice. Brightfield, RFP, DAPI (nuclei localisation) and merged RFP/DAPI images are shown. In Zii, inset shows an enlarged image of an RFP-stained cell located within a crypt and with predicted enterocyte morphology.