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. 2014 Sep 4;7(11):1275–1286. doi: 10.1242/dmm.014720

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Immunohistological characterisation of RFP cells within polyps. Colocalisation experiments were performed on 7-μm frozen sections. (A) Anti-RFP (rabbit polyclonal) and SMA (mouse monoclonal), (B) GLP-1 (goat polyclonal) and SMA (rabbit polyclonal), and (C) RFP (rabbit polyclonal) and E-cadherin (mouse monoclonal). Brightfield, DAPI, red and green fluorescence, and the merged images are shown. RFP cells were found to colocalise with SMA; however, the SMA-labelled cells did not colocalise with either GLP-1 or E-cadherin. Scale bars: 50 μm.