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. 2014 Sep 26;7(11):1287–1296. doi: 10.1242/dmm.016766

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — A HFD induced nitrosative stress in mouse liver. Mice were treated as indicated in Fig. 1. (A) Formalin-fixed, paraffin-embedded sections were deparaffinized and then incubated with anti-3-nitrotyrosine antibody followed by incubation with anti-rabbit IgG FITC-conjugated secondary antibody. Ctr, tyrosine nitrated proteins in the liver of a control mouse. (B) Tyrosine nitrated mitochondrial proteins. Proteins were extracted from hepatic mitochondria and analyzed by western blotting. Membranes were probed with specific antibody against 3-nitrotyrosine (3-NT), and inducible oxide nitric synthase (iNOS). Ponceau S staining shows equal loading of protein per line. MW, molecular weight. (C) 3-tyrosine nitrated proteins in liver mitochondria of a control mouse and a mouse on a HFD. Membrane of a control mouse was first probed with antibody against NDUFB6 (a), ATP5A1 (b), UQCRFS1 (c), MTCO1 (d), MTCO2 (e), COX4 (f) and SDHA (g), and, after removing these antibodies, was probed with specific antibody against 3-NT. Complex A, ATP synthase.