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. 2014 Sep 26;7(11):1287–1296. doi: 10.1242/dmm.016766

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — A HFD upregulates gene expression and activity of NADPHox in mice. NADPHox gene expression (A) and activity (B) was analyzed in mice treated as described in Fig. 1. Data are shown as mean ± s.d. (ns), not significant; *P<0.05; ***P<0.001 versus control mice; [c], P<0.001 versus HFD-fed mice. RLU, relative luminescence units. (C) Phosphorylated Rac1 (Phospho-RAC1) was measured by western blot using a specific antibody. (D) Phosphorylated p47^phox (Phospho-p47^phox) was analyzed after immunoprecipitating (IP) proteins with anti-p47^phox antibody and western blotting (WB) proteins with anti-p47^phox and anti-phosphoserine. MW, molecular weight.