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. 2014 Sep 12;7(11):1307–1315. doi: 10.1242/dmm.016402

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© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Hormonal evaluation of male PANKO-C57 during fasting. The plasma levels of (A) insulin, (B) glucagon and (C) leptin were measured during fasting at 2 and 5 months of age in male PANKO-C57 (KO) and WT mice using the Mouse Metabolic Hormone Panel (Millipore) as measured by the MAGPIX^® Luminex system (n=4–6). (D) Overnight fasting levels of C-peptide from mice aged 2 months using the MAGPIX^® Luminex system as above (n=4). (E) Corticosterone levels were measured from plasma collected during fasting conditions at 2 and 5 months of age using the Corticosterone EIA Kit (Enzo Life Sciences) (n=3). (F) Plasma insulin levels during the course of GTTs (n=3–5). Values are expressed as means±s.e.m. *P<0.05, **P<0.01, ***P<0.001 as determined by using Student’s t-test for A–E and two-way ANOVA in F. IP, intraperitoneal.