Fig. 2.

PHF2 knock-down reduced mRNA and protein levels of adipogenesis marker genes. (A) 3T3-L1 stable cells were differentiated with adipogenesis-inducing media and then lysed at the indicated times. Relative mRNA levels of the indicated genes were determined by qRT-PCR. Bars represents the means ± SDs of three independent experiments, and ^“*” denotes p < 0.01 versus the control shRNA (sh-con) group. (B) Control and PHF2 shRNA-expressing stable 3T3-L1 cell lines were treated with adipogenic rea-gent for the indicated number of days (left) or hours (right), and cell lysates were analyzed with Western blotting with the indicated antibodies.