Figure 2 .

GA induces apoptosis in A2780 cells. Cells were incubated with increasing concentration of GA in RPMI1640 containing 10% FBS for 24h. Then, cells were harvested, washed with cold PBS, incubated with 5µL of Annexin V and 10µL PI for 15 min in dark. 300 µL of binding buffer was added and analyzed by flow cytometry within 1h. Representative data from three independent experiments is shown.