Figure 6. The effects of SLC18B1 gene knockdown on the endogenous expression of SLC18B1 and Spd/Spm in cultured astrocytes.

SLC18B1 gene knockdown was carried out as described in Materials and Methods. (a and b) Gene expression as measured by quantitative PCR (a) and SLC18B1 protein as measured by western blotting analysis (b). Expressions of VAMP2 and V-ATPase subunit A are also shown. (c) Immunohistochemical detection of SLC18B1 protein and Spm/Spd. Bar  =  10 μm.